Vibrational Spectroscopy for Biomedical Applications

One of the primary components of LBRC research has been the development of vibrational spectroscopy and imaging technologies for the diagnosis of diseases including diabetes, atherosclerosis and breast cancer. Three big topics in this core are the following:

  1. Spectroscopic tissue diagnosis [1-5] LBRC pioneered applying optical spectroscopy to human tissues. From diffuse reflectance, intrinsic fluorescence and Raman spectroscopies, disease states of tissues can be monitored through optical fiber probe or imaging device. We are expanding the technique to new clinical applications including middle ear disease diagnosis and tissue identification.
  2. Monitoring cell status and cell-drug interactions [6-8]. Custom-built Raman + QPM system has been successfully applied to various applications. We can monitor morphology as well as chemical composition from live cells without staining. This provides ideal platform to investigate cell status and cell-drug interactions. LBRC is currently improving the speed and resolution of the system for more demanding applications.
  3. Non-invasive blood glucose monitoring by Raman spectroscopy [9-13] LBRC has pioneered the use of NIR Raman spectroscopy for this important diagnostic problem that combines the substantial penetration depth of NIR light with the excellent chemical specificity of Raman spectroscopy. Based on the promising results of small scale volunteer studies, LBRC is currently conducting large scale human study as well as improving the instrument.

Ongoing Projects

  1. Development of live cell assay using SERS [PDF]
  2. Spectroscopic diagnosis of middle ear diseases [PDF]
  3. Chemical profiling for theranostics applications [PDF]
  4. White adipose tissue inflammation
  5. Spectroscopy-guided needle procedure

Background Publications

  1. Zonios G, Perelman LT, Backman V, Manoharan R, Fitzmaurice M, Van Dam J, Feld MS, Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo. Appl Opt, 38(31): p. 6628-37 (1999).
  2. Mueller MG, Georgakoudi I, Zhang Q, Wu J, Feld MS, Intrinsic fluorescence spectroscopy in turbid media: disentangling effects of scattering and absorption. Appl Opt, 40(25): p. 4633-46 (2001).
  3. Haka AS, Shafer-Peltier KE, Fitzmaurice M, Crowe J, Dasari RR, Feld MS, Diagnosing breast cancer by using Raman spectroscopy. Proc Natl Acad Sci U S A, 102(35): p. 12371-6 (2005).
  4. Pandey R, Paidi SK, Kang JW, Spegazzini N, Dasari RR, Valdez TA, Barman I. Discerning the differential molecular pathology of proliferative middle ear lesions using Raman spectroscopy. Scientific Reports, 5: p. 13305 (2015).
  5. Anderson TA, Kang JW, Gubin T, Dasari RR, So PTC. Raman Spectroscopy Differentiates Each Tissue from the Skin to the Spinal CordA Novel Method for Epidural Needle Placement? Anesthesiology, 125(4); p. 793-804 (2016).
  6. Kang JW, Nguyen FT, Lue N, Dasari RR, Heller DA, Measuring uptake dynamics of multiple identifiable carbon nanotube species via high-speed confocal Raman imaging of live cells. Nano Letters, 12(12): p. 6170-6174 (2012).
  7. Li M, Kang JW, Sukumar S, Dasari RR, Barman I. Multiplexed detection of serological cancer markers with plasmon-enhanced Raman spectro-immunoassay. Chemical Science, 6(7): p. 3906-3914 (2015).
  8. Kang JW, So PTC, Dasari RR, Lim DK. High Resolution Live Cell Raman Imaging Using Subcellular Organelle-Targeting SERS-Sensitive Gold Nanoparticles with Highly Narrow Intra-Nanogap. Nano Letters, 15(3): p. 1766-1772 (2015).
  9. Berger AJ, Koo TW, Itzkan I, Horowitz G, Feld MS. Multicomponent blood analysis by near-infrared Raman spectroscopy. Applied Optics. 38, 2916-2926 (1999).
  10. Enejder AMK, Koo TW, Oh J, Hunter M, Sasic S, Feld MS, Horowitz GL. Blood analysis by Raman spectroscopy. Optics Letters. 27, 2004-2006 (2002).
  11. Barman I, Kong CR, Singh GP, Dasari RR, Feld MS. Accurate spectroscopic calibration for non-invasive glucose monitoring by modeling the physiological glucose dynamics. Analytical Chemistry. 82, 6104-6114 (2010).
  12. Kong CR, Barman I, Dingari NC, Kang JW, Galindo L, Dasari RR, Feld MS, A novel non-imaging optics based Raman spectroscopy device for transdermal blood analyte measurement, AIP Advances, 1, 032175 032175-13 (2011).
  13. Spegazzini N, Barman I, Dingari NC, Pandey R, Soares JS, Ozaki Y, Dasari RR. Spectroscopic approach for dynamic bioanalyte tracking with minimal concentration information. Scientific Reports, 4: p. 7013 (2014).